Fig. 5 | Scientific Reports

Fig. 5

From: Dapagliflozin ameliorates high glucose-induced peritoneal fibrosis through downregulation of ENKUR/PI3K/AKT signaling pathway

Fig. 5

DAPA attenuates EMT of HMrSV5 stimulated by high glucose by inhibiting ENKUR/PI3K/AKT pathway. (A) Cell viability of HMrSV5 treated with different concentrations of DAPA (0,0.1,1,5,10,20,30 µ M) under high glucose (HG) conditions was determined by CCK8 assay. (B,C) Treated with DAPA (0,0.1,1,5 µ M) and exposed to HG, the protein and gene expression levels of E-cadherian, α-SMA, COL-IV, ENKUR and PI3K/AKT pathway in HMrSV5 cells treated with DAPA (0,0.1,1,5 µ M) and exposed to HG were analyzed by Western blot and qRT-PCR.**p < 0.01, *** p < 0.001 for ANOVA. Uncropped gels and blots were included in a Supplementary Information file.

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