Fig. 4

miR-1285-3p targeted G6PI and negatively modulated the expression of G6PI in TNBC. (A) The putative binding between miR-1285-3p with the 3’-UTR of G6PI predicted using the miRDB online site. (B) TNBC cells were transfected with miR-NC or miR-1285-3p with the WT or Mut 3’-UTR of G6PI. The luciferase activity was determined. (C) miR-1285-3p transfection in TNBC cells inhibited the expression levels of G6PI. (D) Knockdown of LINC02159 reduced the mRNA abundance of G6PI in TNBC cells. The experiments in (B–D) were performed with 3 biological replicates. (E) Protein expression of G6PI in TNBC cells transfected with siRNA-LINC02159 was inhibited compared with cells carrying siRNA-control. The whole blot was provided in supplementary information file. The experiment was performed with 3 biological replicates, and one representative data was shown.