Fig. 6 | Scientific Reports

Fig. 6

From: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis

Fig. 6

LINC02159 modulated the proliferation and glycolysis of TNBC cells via G6PI. (A) Flag tagged G6PI was transfected into TNBC cells to rescue the expression of endogenous G6PI. The whole blot was provided in supplementary information file. (B,C) Transfection of G6PI obviously attenuated the suppressive role of LINC02159 depletion on the proliferation of TNBC cells. (D,E) The glucose uptake and lactate generation were rescued with the overexpression of G6PI in LINC02159-depleted TNBC cells. (F,G) Seahorse tracing curves of TNBC cells showed that transfection of G6PI rescued the glycolysis flux and glycolytic capacity of TNBC cells induced by LINC02159 depletion. The above experiments were performed with 3 biological replicates.

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