Fig. 3

Choline and ethanolamine transport and CTL1 transporter are upregulated in PCYT2 knockdown cells. The uptake of (A) [³H]Cho and (B) [¹⁴C]Etn shows that both transports were significantly increased in KD cells relative to WT cells. (C) The expression of CTL1 gene SLC44A1 was significantly reduced at mRNA level in KD cells relative to WT cells. (D,E) At the protein level, CTL1 was expressed 3-folds more in KD cells. (F,G) The treatments with 2.5 and 5 mg/L of Cho increased the CTL1 protein in both cell types in a concentration dependent manner. The expression of CTL2 gene SLC442 mRNA (H) and CTL2 protein (I,J) were the same in KD and WT cells. Cho reduced CTL2 in WT cells (K) and elevated CTL2 in KD cells (L). The experiments are expressed as means ± SEM and significant differences between treatments were compared by one-way ANOVA with post-hoc Bonferroni or Tukey’s test to compare means between WT untreated and Cho treated, and KD untreated and Cho treated groups, as indicated by (*P < 0.05), (**P < 0.005) or (#P < 0.001). The results shown are from three individual experiments.