Fig. 4
The regulation of CDP-choline pathway and PCYT1. (A) WT and KD cells were incubated 24 h with [3H]Cho and the water-soluble intermediates [3H]Cho, [3H]PCho, and [3H]CDP-Cho and the final product [3H]PC ‘pool sizes’ were compared. (B) The western blots of the CDP-Cho pathway regulatory enzyme PCYT1 were determined at 0 and 2.5 mg/L Cho. The relative changes in PCYT1 protein at the basal level (C) and after 21-day treatment with Chol were compared in WT (D) and KD (E) cells. Differences in PCYT1 protein were determined by one-way ANOVA with post-hoc Bonferroni or Tukey’s test to compare means between WT and KD cells (C), WT untreated and WT Cho treated, (D) and KD untreated and KD Cho treated groups (E). The results shown are from three individual experiments. The significant differences are indicated by *(P < 0.05) and **(P < 0.005) and *** (P < 0.001) .
