Fig. 3
From: PKA prevents the resection of DNA double-strand breaks and favours nonhomologous end-joining
Effect of PKA on 53BP1 foci assembly after ionizing radiation (IR). (A) Representative image of nuclear 53BP1 foci after IR (right panel). Scale bar: 10 µm. (B) Kinetics of 53BP1 foci assembly after IR (2 Gy). Upper panel: silencing of PKAcs with siRNA. Lower panel: quantification of 53BP1 (means ± SEMs) foci assembly at different times after IR, with siCTRL (sicontrol) or siPKAcs (20 nM). The data represent at least 3 independent experiments (p values: * < 0.05). (C) Kinetics of pCREB phosphorylation after IR. (D) Effect of the PKA inhibitor H89 on IR-induced pCREB. Uncropped gels are shown in supplementary data.
