Table 2 Variability analysis of motor neuron differentiations per quantitative QC metric as performed in R; R2 values represent percentage of variability attributable to “Cell Line”, “Induction Set” or “Operator” per QC metric where “-” indicates no significant contribution; adjusted R-squared values indicate strength of linear model (range 0–1, adjusted R-squared > 0.60 acceptable).

From: Examining iPSC derived motor neuron variability and genome stability monitoring as a solution

QC Metric

Coefficient of variance (%)

R² - Explanatory power (%)

Linear Model Diagnostics

Cell Line

Induction Set

Operator

Adjusted R-squared

NPC: D3

59.5

-

-

67.1

0.77

D3:D10

67.0

31.5

-

31.4

0.63

D10 Neurite Area (µm²)

53.7

7.1

-

-

0.65

PAX6 + OLIG2 (NPC)

46.3

1.5

51.1

-

0.96

SMI32 + MAP2 (D3)

46.5

9.7

42.5

-

0.84

SMI32 + MAP2 (D10)

36.8

6.3

57.2

-

0.60

ISL1 + MAP2 (D3)

36.8

11.2

45.4

39.6

0.87

ISL1 + MAP2 (D10)

25.1

-

-

-

-

  1. QC = Quality control; NPC = Neural precursor cell; D3 or D10 = motor neurons terminally differentiated for 3 or 10 days from neural precursor stage; PAX6, OLIG2 = neural precursor marker; SMI32, ISL1 = mature motor neuron markers; MAP2 = post mitotic neuron marker.
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