Table 3 Variability and QC data for differentiations performed on iPSCs where chromosomal stability status was known; “All sets” include cell lines with and without abnormalities whilst “No detectable abnormalities” only includes data from the latter, as confirmed via a bulk qPCR assay targeting common iPSC chromosomal abnormalities; coefficient of variance data highlighted in bold indicates an improvement in value; QC metrics as previously defined; * = p < 0.05, ns = p > 0.05, as determined by a student’s T-test.
From: Examining iPSC derived motor neuron variability and genome stability monitoring as a solution
QC Metric | Coefficient of variance (%) | QC Metric Value | QC Metric Significance Testing (All sets vs. no detectable abnormalities) | |||
|---|---|---|---|---|---|---|
All sets | No detectable abnormalities | All sets | No detectable abnormalities | T-value | p-value | |
NPC: D3 | 32.14 | 35.61 | 3.22 | 2.85 | - | n.s. |
D3:D10 | 49.16 | 36.44 | 1.65 | 1.28 | - | n.s. |
D10 Neurite Area | 20.18 | 20.59 | 23212.12 μm² | 26593.58 μm² | - | n.s. |
PAX6 + OLIG2 (NPC) | 13.57 | 1.81 | 80.83% | 94.25% | 1.45 | * |
SMI32 + MAP2 (D3) | 18.64 | 7.09 | 71.17% | 87.25% | 1.41 | * |
SMI32 + MAP2 (D10) | 32.34 | 20.16 | 55.67% | 66.50% | - | n.s. |
ISL1 + MAP2 (D3) | 16.25 | 8.33 | 70.67% | 86.75% | 1.62 | * |
ISL1 + MAP2 (D10) | 28.27 | 16.36 | 56.50% | 51.50% | - | n.s. |
