Table 4 Chromosomal copy number as assessed via bulk qPCR assay; five rounds of differentiation completed across two cell lines; One-way ANOVA performed comparing copy number against all loci; copy number < 1.5 or > 2.5 (< 0.7 or > 1.3 for chromosome X for lines from male donors) and p < 0.05 with post hoc Tukey adjustment indicate abnormality.
From: Examining iPSC derived motor neuron variability and genome stability monitoring as a solution
Cell Line | Differentiation Round | Detected Abnormalities | Copy Number | Adjusted p-value | Abnormal? |
|---|---|---|---|---|---|
WTSli010-A | 1,2 | Chr1q | 1.51 | 0.93 | ~ |
3,4 | Chr8q, Chr17q, ChrXp | 1.23, 1.17, 0.68 | 0.68, 0.64, 0.03 | ✓ | |
5 | - | - | - | - | |
WTSli022-A | 1,2 | Chr18q | 0.65 | 0.65 | ~ |
3,4 | - | - | - | - | |
5 | Chr18q | 1.38 | 0.26 | ~ |