Fig. 5

Association of the CS subgroups with immune infiltration levels and immunophenotypic features. (A) Multiplex immunohistochemistry staining of CD45 (red), MYC (cyan), PD-L1 (pink), FABP4 (yellow), and DAPI (blue) in tissue microarrays of cancer patients. Scale bars represented 200 μm. (B–D) The lung and liver tissues from young (6–8 weeks) or old (20–22 months) C57BL/6 mice were used for flow cytometry analysis (n = 6 mice per group). Data were presented as mean ± standard error of mean (SEM). Unpaired t-test. (B) Representative scatterplots of CD8⁺/CD4⁺ naive T (Tnaive, CD44⁻ CD62L⁺), effector memory T (Tem, CD44⁺ CD62L⁻), and central memory T (Tcm, CD44⁺ CD62L⁺) cells from CD45⁺ CD3⁺ CD8⁺/CD4⁺ cells. (C) The proportions of CD8⁺/CD4⁺ T, Tnaive, Tem, Tcm, CD69⁺ T, and PD-1⁺ T cells in CD45⁺ cells. (D) The proportions of neutrophils (CD45⁺ CD11c⁻ CD11b⁺ Ly6G⁺), macrophages (CD45⁺ CD11c⁻ CD11b⁺ F4/80⁺), conventional dendritic cells (CD45⁺ CD11c⁺ MHCII⁺), and alveolar macrophages (CD45⁺ CD11c⁺ CD11b⁻ F4/80⁺) in CD45⁺ cells. (E) Establishement of orthotopic LUAD models in young (6–8 weeks) or old (20–22 months) C57BL/6 mice. (F) Comprision of cytokine levels in bronchoalveolar lavage fluid of young and old mice with LUAD (n = 3 mice per group). Multiple t-tests adjusted by Holm-Sidak method.