Fig. 2
Immune cell composition in E. muris-infected colons. (A, B) Representative immunohistochemistry (IHC) images and quantification of CD45, CD11c, CD4, F4/80, CD3, and FOXP3-expressing cells in colon tissues of E. muris-infected at 9 dpi and 45 dpi and uninfected mice. Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ***P < 0.001 (t-test). (C) Colonic inflammation score assessed at 14dpi by the scoring system. Statistical significance was determined using t-tests: ***P < 0.001(D) Flow cytometric analysis for quantifying the proportions of immune cell populations in the colonic lamina propria at 14 days post–E. muris infection. Shown are the proportions of CD3⁺ T cells, CD11c⁺ dendritic cells, F4/80⁺ macrophages, CD11b⁺ myeloid cells, CD19⁺ B cells, and Ly6G⁺ neutrophils among CD45⁺ cells in uninfected and infected BALB/c mice. Data represent mean ± SD; each dot represents an individual mouse. (E) qPCR analysis of colonic tissues from infected and uninfected mice at 14 dpi. Expression levels of Il4, Il5, Tnf, Il1b, Il17a, and Il10 were measured. *P < 0.05 (t-test).
