Fig. 4
The alterations in colonic epithelial cells during E. muris infection. (A, B) Representative images for Ki-67 and quantification of positive cells in colon tissues of E. muris-infected and uninfected mice at 9 dpi and 45 dpi. Scale bar: 200 μm–50 μm for enlarged. The height of Ki-67 + cell distribution was measured along with the height of the colonic glands. **P < 0.01, and ***P < 0.001. (C, D) Representative AB-PAS-stained images and quantification of AB-positive goblet cell number in colon tissues of E. muris-infected (9 dpi, 45 dpi) and uninfected mice. Scale bar: 200 μm–50 μm for enlarged. Goblet cells were indicated by blue-colored droplets. (E) The graphs depict the correlations between inflammation scores and the number of Ki-67-positive cells (proliferating cells) per unit area or the number of goblet cells (mucin-producing cells) per unit area.
