Fig. 3 | Scientific Reports

Fig. 3

From: Effects of female hormone withdrawal and ulipristal acetate on MED12 mutation positive and negative uterine leiomyomas using a xenograft model

Fig. 3

Detection of apoptotic cells in xenograft tumors by TUNEL staining. The images show the representatives in the xenograft tumors of the E(-)P(-) and EP + UPA groups in both ULM subtypes 8 weeks after transplantation. Rat mammary tissue was used as a positive control to detect the apoptotic cells. A sample of TdT enzyme withdrawal in the EP + UPA group (EP + UPA_TdT enzyme (-)) was used as a negative control to detect the apoptotic cells. This analysis used three specimens in each MED12m (+)- and (-)-ULM. In TUNEL staining, apoptotic cells were detected as the cells that bore dark red-brown nuclei (arrowheads). Scale bar = 50 μm. Because almost no apoptotic cells were detected in E(+)P(+) and E(+)P(-) groups in both ULM subtypes, their images were omitted.

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