Table 4 Arena, conditions and endpoints collected for all diet bioassays performed with Cry1B.2, Cry1B.3, Cry1Da_7 and Vip3Cb1.

From: Characterization of the spectrum of insecticidal activity of Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins produced by lepidopteran-protected MON 89151 cotton

Common name (insect code)

Order: Family

Protein tested

Endpoints

Bioassay duration (days)

Arena used; target number of insects infested per in-assay replicate

Bioassay conditions

Soybean looper (SBL)1

Lepidoptera: Noctuidae

Cry1B.2

Survival and body mass

7

128-well tray; 16

27 °C, 60% RH, 14:10 h light:dark

Fall armyworm (FAW)1

Lepidoptera: Noctuidae

Cry1B.2

Cry1B.3

Cry1Da_7

Vip3Cb1

Survival and body mass

7

128-well tray; 16

27 °C, 60% RH, 14:10 h light:dark

Corn earworm (CEW)2

Lepidoptera: Noctuidae

Cry1B.2

Cry1B.3

Cry1Da_7

Vip3Cb1

Survival and body mass

7

128-well tray; 16

27 °C, 60% RH, 14:10 h light:dark

European corn borer (ECB)1

Lepidoptera: Crambidae

Cry1B.2

Cry1B.3

Cry1Da_7

Vip3Cb1

Survival and body mass

7

128-well tray; 24

27 °C, 60% RH, 14:10 h light:dark

Velvetbean caterpillar (VBC)1

Lepidoptera: Erebidae

Cry1B.2

Survival and body mass

7

128-well tray; 24

27 °C, 60% RH, 14:10 h light:dark

Tobacco budworm (TBW)2

Lepidoptera Noctuidae

Vip3Cb1

Survival and body mass

7

128-well tray; 24

27 °C, 60% RH, 14:10 h light:dark

Colorado potato beetle (CPB)3 (Cry1B.2, Vip3Cb1), 6 (Cry1Da_7)

Coleoptera: Chrysomelidae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival and body mass (only for Vip3Cb1 and Cry1B.2)

7 (Cry1B.2, CryDa_7), 6 (Vip3Cb1)

128-well tray; 24

27 °C, 60% RH, 14:10 h light:dark

Southern corn rootworm (SCR)3 (Cry1Da_7), 7 (Cry1B.2, Vip3Cb1)

Coleoptera: Chrysomelidae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival and body mass (only for Cry1B.2 and Vip3Cb1)

7

48-well plate;

32 (Cry1B.2, Cry1Da_7), 30 (Vip3Cb1)

25 °C, 70% RH, 0:24 h light:dark (Cry1Da_7, Vip3Cb1), 27 °C, 70% RH, 0:24 h light:dark (Vip3Cb1, Cry1B.2)

Western corn rootworm (WCR)3 (Cry1Da_7, Cry1B.2), 7 (Vip3Cb1)

Coleoptera: Chrysomelidae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival and body mass (only for Cry1B.2 and Vip3Cb1)

7

48-well plate; 32 (Cry1B.2, Cry1Da_7), 30 (Vip3Cb1)

25 °C, 70% RH, 0:24 h light:dark

Mexican bean beetle (MBB)4

Coleoptera: Coccinellidae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival and body mass (only for Cry1B.2 and Vip3Cb1)

7

128-well tray; 24

27 °C, 60% RH, 14:10 h light:dark

Neotropical brown stink bug (NBS)3

Hemiptera: Pentatomidae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival

5

128-well tray; 32

27 °C, 60% RH, 14:10 h light:dark (Cry1Da_7), 27 °C, 80% RH, 14:10 h light: dark (Cry1B.2), 27 °C, 80% RH, 16:8 h light: dark (Vip3Cb1)

Western tarnished plant bug (WTP)3 (Cry1B.2, Vip3Cb1), 8 (Cry1Da_7)

Hemiptera: Miridae

Cry1B.2

Cry1Da_7

Vip3Cb1

Survival

6

128-well tray; 32

27 °C, 60% RH, 14:10 h light:dark (Vip3Cb1, Cry1Da_7), 27 °C, 80% RH 14:10 h light:dark (Cry1B.2)

Common fruit fly (CFF)5

Diptera: Drosophilidae

Cry1B.2

Vip3Cb1

Survival (Cry1B.2, Vip3Cb1) and development time to pupa (Vip3Cb1)

10 (Cry1B.2), 12 (Vip3Cb1)

48-well plate; 30

25 °C, 70% RH, 16:8 h light:dark (Vip3Cb1), 25 °C, 70% RH, 14:10 h light:dark (Cry1B.2)

  1. Insect Source: 1Bayer CropScience LP (Union City, TN); 2Benzon Research Inc. (Carlisle, PA); 3Bayer CropScience LP (Waterman, IL); 4New Jersey Dep. Of Agriculture (Trenton, NJ); 5Carolina Biological Supply (Burlington, NC); 6French Agricultural Research, Inc., (Lamberton, MN); 7Crop Characteristics, Inc. (Farmington, MN); 8USDA-ARS (Stoneville, MS).
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