Table 1 Yeast strains used in this study.
From: Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae
Abbreviation | Strain | Nuclear genotype | Mitochondrial genotype | Source |
|---|---|---|---|---|
WT | w303a-187 | MATa ade2 leu2 his3 ura3 trp1 can1 MHR1 | ρ+ ω− ens2 Chl321R | |
mhr1-1 | FL672C-55R5 | MATa leu2 trp1 can1 mhr1-1 | ρ+ ω+ ens2 Oli2R | |
WT pVT-mtTomato | w303a-187 pVT100U-mtTomato | MATa ade2 leu2 his3 ura3 trp1 can1 MHR1 pVT100U (mtTomato, URA3) | ρ+ ω− ens2 Chl321R | Stock culture |
WT ρ+ pVT-mtTomato | YKN1423 pVT100U-mtGFP | MATα leu2 ura3 met3 MHR1 | ρ+ ω+ ens2 Oli2R | This study |
mhr1-1 pVT-mtTomato | AFS98-4a pVT100U-mtTomato | MATa ade3 leu2 his3 ura3 trp1 can1 ::GPD-HSVTK mhr1-1 pVT100U (mtTomato, URA3) | ρ+ | This study |
WT ρ0 pVT-mtTomato | w303a-187 ρ0 pVT100U-mtTomato | MATa ade2 leu2 his3 ura3 trp1 can1 pVT100U (mtTomato, URA) | ρ0 | Derived from w303a-187 by treatment with ethidium bromide |
WT pVT-MHR1 | w303a-187 pVT100U-MHR1 | MATa ade2 leu2 his3 ura3 trp1 can1 MHR1 pVT100U (MHR1, URA3) | ρ+ ω− ens2 Chl321R | This study |
WT HS ρ− | HSC1 | MATα leu2 ura3 met3 MHR1 | HS [ori5] ρ− mtDNA | |
Δmhr1 HS ρ− | HSC 1 Δmhr1 | MATα ura3 met3 mhr1 | HS [ori5] ρ− mtDNA | Stock culture |
WT HS ρ− pVT-mtGFP | HSC1 pVT100U-mtGFP | MATα leu2 ura3 met3 MHR1 pVT100U (mtGFP, URA3) | HS [ori5] ρ− mtDNA | Stock culture |
Δmhr1 HS ρ− pVT-mtGFP | HSC1 Δmhr1 pVT100U-mtGFP | MATα ura3 met3 mhr1 pVT100U (mtGFP, URA3) | HS [ori5] ρ− mtDNA | This study |
WT ρ0 pVT-mtGFP | HSC1 ρ0 pVT100U-mtGFP | MATα leu2 ura3 met3 pVT100U (mtGFP, URA3) | [ρ0] | Derived from HSC1 by treatment with ethidium bromide |
WT HS ρ− pVT-MHR1 | HSC1 pVT100U-MHR1 | MATα leu2 ura3 met3 MHR1 pVT100U (MHR1, URA3) | HS [ori5] ρ− mtDNA | This study |
WT HS ρ− pVT-MHR1 pVT-mtGFP | HSC1 pVT100U-MHR1 pVT100L-mtGFP | MATα leu2 ura3 met3 MHR1 pVT100U (MHR1, URA3) pVT100L (mtGFP, LEU2) | HS [ori5] ρ− mtDNA | This study |
