Fig. 2
Lung histology in pendrin-KO mice after 5-h HTV ventilation showed a significantly lower degree of leukocyte infiltrations and lung injury score. Pendrin wild-type (WT) and knockout (KO) mice were subjected to a tracheostomy and ventilation with a supine or prone position at a high tidal volume (HTV) of 30 mL/kg. Pendrin inhibitor (YS-01) was administered intra-peritoneally 1 h before inducing VILI in the supine position. (A) Lung injury scoring of each group. (B) Histopathologic image of hematoxylin and eosin (H&E) staining (× 400). Representative images are shown; minor variations in exposure do not affect data interpretation. NVC, non-ventilation control group; supine HTV, HTV ventilation in supine position; prone HTV, HTV ventilation in prone position. *p < 0.05, **p < 0.01, ***p < 0.001 analyzed by Student’s unpaired two-tailed t-test.
