Fig. 4
Pendrin expression increases in HTV ventilation mice. Pendrin wild-type (WT) and knockout (KO) mice were conducted to a tracheostomy and ventilation with a supine or prone position at a high tidal volume (HTV) of 30 mL/kg. Pendrin inhibitor (YS-01) was administered intra-peritoneally 1 h before inducing VILI in the supine position. (A) Western blot and (B) densitometry analyses of pendrin bands. Cropped images are shown in figure for clarity. The original gels with visible edges are presented in Supplementary Fig. S1. NVC, non-ventilation control group; supine HTV, HTV ventilation in supine position; prone HTV, HTV ventilation in prone position; PDS, pendrin. *p < 0.05, **p < 0.01, ***p < 0.001 analyzed by Student’s unpaired two-tailed t-test.
