Fig. 5
Effect of pendrin deletion on VILI as demonstrated by transmission electron micrographic immune-gold labeling. Pendrin wild-type (WT) and knockout (KO) mice were subjected to tracheostomy and ventilation with a supine or prone position at a high tidal volume (HTV) of 30 mL/kg. (A) Non-ventilated group, pendrin-WT mice. (B) Non-ventilated group, pendrin-KO mice. (C) HTV ventilation in the supine position, pendrin-WT mice. (D) HTV ventilation in the supine position, pendrin-KO mice. (E) HTV ventilation with YS-01 before 1 h in the supine position. (F) HTV ventilation in the prone position, pendrin-WT mice. (G) HTV ventilation in the prone position, pendrin-KO mice. (H) Comparisons of gold particle count labeled with pendrin. Black triangles mark regions of immune-gold particle detection. BEC, bronchial epithelial cell; BL, bronchial lumen; C, cilia. *p < 0.05, **p < 0.01, ***p < 0.001 analyzed by Student’s unpaired two-tailed t-test.
