Fig. 4

CircPSMA4 promotes the Warburg effect and migration/invasion of bladder cancer cells through miR-767-3p. (A) The effects of circPSMA4 overexpression and miR-767-3p interference on prostate cancer cell activity were analyzed through CCK-8 experiments; (B) The effects of circPSMA4 overexpression and miR-767-3p interference on prostate cancer cell proliferation were analyzed through a colony formation assay; (C) The effects of circPSMA4 overexpression and miR-767-3p interference on the migration and invasion abilities of prostate cancer cells were analyzed through Transwell experiments; (D) The effects of circPSMA4 overexpression and miR-767-3p interference on the migration ability of prostate cancer cells were analyzed through scratch assay; (E,F) The effects of circPSMA4 overexpression and miR-767-3p interference on prostate cancer cell behavior were analyzed through lactic acid detection and glucose uptake experiments; (G,H) Seahorse energy metabolism analysis showing circPSMA4’s effect on glycolysis and mitochondrial respiration of BCa cells. (I) Western blot was used to detect the protein expression levels of HK2, GLUT1, and LDHA in the OE-NC group, OE-circPSMA4 group, OE-circPSMA4 + miR-NC mimics group, and OE-circPSMA4 + miR-767-3p mimics group; (J) Evaluation of the effects of circPSMA4 and miR-767-3p on cellular ATP concentration. N = 3;**P < 0.01;*P < 0.05.