Fig. 6
3.6 Silibinin Delayed AKI-to-CKD Progression by Modulating PI3K/AKT Signaling, Oxidative Stress, Inflammation, and Apoptosis. (A-D). Measurement of Cr, BUN, H2O2, MDA production in each groups. (E, F, J). qRT-PCR analysis of mRNA expression of TNF-α, IL-1β, IL-6 in each groups. (G-I, L-O). Western blot and quantitative analysis of TNF-α, IL-1β, IL-6, Bax, cleaved caspase-3, Caspase-3 Bcl-2, p-PI3K, PI3K, p-AKT, AKT in each groups. (O, P). H&E, Masson’s trichrome, Sirius red, TUNELstaining(and its quantitative analysis) in each groups. Each experiment was repeated independently for a minimum three times. Data: mean ± SEM (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham; #P < 0.05, ##P < 0.01, ###P < 0.001vs. IRI 14d. (one-way ANOVA with Tukey’s post hoc test).
