Fig. 2

In vitro effects of the exposure to rhein on the expression of FTO, IRX3, PPARα and AMPKα in ZFL cells after 2 h and 24 h of DMSO 1% (control) or containing 0. 1, 1 or 10 µM of Rhein (A-D). Representative blots for AMPKα, FTO, IRX3 and PPARα in ZFL cells (E) and protein expression of AMPKα, FTO, IRX3 and, PPARα (F-I) after 24 h exposure to 1% DMSO (control) or 0. 1, 1 or 10 µM rhein. The corresponding full, uncut blots are presented in Supplementary Material - Fig. 3. Data were assessed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test. Results are expressed as mean ± SEM. Asterisks indicate significant differences between the control and treated groups * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Black lines under the asterisks cluster groups with same significance when compared to controls. Each group consisted of six samples, derived from two independent studies. Fat mass and obesity associated (FTO), Iroquois homeobox protein (IRX3), proliferator-activated receptor alpha (PPARα) and AMP‐activated protein kinase (AMPKα).