Fig. 3

In vivo effects of rhein on FTO, PPARα and AMPKα. Representative western blot images showing FTO and β-actin in the liver of zebrafish 1 h after the intraperitoneal administration of 1% DMSO (control) or 1, 10 or 100 ng/g bw of rhein (A). The corresponding full, uncut blots are presented in Supplementary Material - Fig. 4. Quantitative data from the analysis of the above blots showing FTO protein (B). FTO mRNA, IRX3 mRNA, PPARα mRNA, AMPKα mRNA and LEPRb abundance in zebrafish liver (C-G) 1 h after intraperitoneal administration of 1% DMSO (control) or 1, 10 or 100 ng g-1 body weight rhein. Data are expressed as mean + SEM (n = 6). Data were assessed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test., * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Black lines under the asterisks cluster groups with same significance when compared to controls. Fat mass and obesity associated (FTO), Iroquois homeobox protein (IRX3), proliferator-activated receptor alpha (PPARα) and AMP‐activated protein kinase (AMPKα).