Fig. 6

Effects of rhein on feeding regulation in zebrafish. Relative FTO mRNA abundance in brain of zebrafish (a), Food intake (b) 1 h after the intraperitoneal administration of 1% DMSO (control) or containing 1, 10 or 100 ng g⁻¹ body weight rhein. The pre-injection period is presented as the mean daily food intake derived from the one-week baseline monitoring of feeding prior to treatment, while the injection-day values indicate the amount of food intake within 1 h after injection on the day of administration. IRX3 mRNA, Orexin mRNA and NPY mRNA abundance (C-E), Representative blots for FTO, IRX3, Orexin and NPY in the brain of zebrafish (F) and protein expression of FTO, IRX3, Orexin A and NPY in the brain of zebrafish (G-J) 1 h after intraperitoneal administration of 1% DMSO (control) or containing 1, 10 or 100 ng/g bw rhein. The corresponding full, uncut blots are presented in Supplementary Material - Fig. 5. Data were assessed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisonstest. Results are expressed as mean + SEM. Asterisks indicate significant differences between the control and treated groups * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Black lines under the asterisks cluster groups with same significance when compared to controls. Each group consisted of six samples, derived from two independent studies. Fat mass and obesity associated (FTO), Iroquois homeobox protein (IRX3) and Neuropeptide Y (NPY).