Fig. 4 | Scientific Reports

Fig. 4

From: Design, synthesis, and biological evaluation of novel 6-(4-aminopiperidin-1-yl)-substituted benzyl-3-methylpyrimidine-2,4(1H,3H)-dione derivatives as potential anticancer agents targeting thymidylate synthase

Fig. 4

Flow cytometry analysis of apoptosis induction by compound 5 h in MCF-7 cells was performed using Annexin V-FITC/PI dual staining. Cells were treated with 0 (control), 8, 16, and 32 µM concentrations of 5 h for 72 h. Representative dot plots illustrate the distribution of viable (Annexin V⁻/PI⁻), early apoptotic (Annexin V⁺/PI⁻), late apoptotic (Annexin V⁺/PI⁺), and necrotic (Annexin V⁻/PI⁺) cells.

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