Fig. 4
NSC23766 suppressed VEGF165-induced cytoskeletal reorganization in HRMECs via the LIMK/cofilin signaling pathway. (A) Fluorescence microscopy images showing F-actin staining (phalloidin, green) and nuclei (DAPI, blue) in HRMECs. White arrows indicate VEGF165-induced F-actin reorganization and its modulation by NSC23766 treatment. Scale bar: 10 μm. (B) the Western blotting assays test the protein expression of p-LIMK, LIMK, p-cofilin, cofilin and β-actin in HRMECs after treatment with VEGF165 (20 ng/mL) for 10 min and NSC23766 (200 µmol/mL) (original blots are presented in Supplementary Material 1). (C) Quantification and statistical analysis of F-actin fluorescence intensity (green channel) in (A) (D) Quantification and statistical analysis of Western blot band intensities for p-LIMK/total LIMK ratio are shown in (B) (E) Quantification and statistical analysis of Western blot band intensities for p-cofilin/total cofilin ratio are shown in B. Data are presented as mean ± SEM. *: P < 0.05, **: P < 0.01, ***: P < 0.001, multiple-group comparisons were analyzed by one-way ANOVA.
