Fig. 2

Immunohistochemical staining of PCNP in HCC tissues than the adjacent tissues. A Representative IHC stain in HCC tissue array. Images of three representative patients were taken under amplification of 2 or 40. B The immunoreactive score (IRS) in cytoplasmic or nucleus fractions were calculated as intensity of the staining reaction multiplied by the percentage of positive cells. C relative PCNP expression levels in HCC cell lines were measured by performing RT-qPCR using cDNA microarray, including SNU449, Hep3B, SK-HEP-1, PLC-PRF-5, RBE, Huh7 and non-tumor cell line L-02. D The prognostic value of PCNP protein levels in cytoplasmic or nucleus faction on LIHC were analyzed.