Fig. 12
Immunofluorescence Staining of PDK3 Expression in Cancer Cells Treated with p-C). (A) Cancer cells were treated with increasing concentrations of p-C (5 µM, 10 µM, 20 µM, 50 µM, and 100 µM) and then subjected to immunofluorescence staining using an anti-PDK3 primary antibody, followed by a fluorescently labelled secondary antibody. The control group showed robust PDK3 expression, as indicated by strong green fluorescence throughout the cytoplasm. In contrast, treatment with higher concentrations of p-C (50 µM and 100 µM) resulted in a significant reduction in PDK3 expression, as evidenced by the decreased fluorescence intensity. At these higher doses, PDK3 expression was notably diminished, suggesting a dose-dependent inhibitory effect of p-C on PDK3. Cells treated with lower p-C concentrations (5 µM and 10 µM) displayed moderate PDK3 expression, but still lower than the control. Images were captured using a Zeiss microscope at 20X magnification. Scale bar: X µm. (B) fluorescence images showing PDK3 expression after treatment. Quantification demonstrated that p-C at 10, 20, 50, and 100 µM significantly reduced PDK3 expression compared to control (**p < 0.01 and *p < 0.05). Data are expressed as mean ± SEM; n = 3; statistical analysis by Tukey’s multiple comparison test.
