Fig. 4 | Scientific Reports

Fig. 4

From: Optimization of primary culture, inflammatory induction, and molecular analysis of fibroblast-like synoviocytes in a rat model of osteoarthritis pain

Fig. 4

Time- and dose-dependent induction of IL-1β and TNF-α by LPS in FLS. (A) Representative Western blots of IL-1β and TNF-α protein expression. (B, C) Quantitative analysis showing peak expression at 3 h: (B) IL-1β (F = 28.7, P < 0.001), (C) TNF-α (F = 175.0, P < 0.001). (D, E) RT-qPCR analysis of (D) IL-1β and (E) TNF-α mRNA demonstrating maximal induction at 1000 ng/mL for 3 h. Data are presented as mean ± SD of 5 biological replicates; * P < 0.05, **P < 0.01, ***P < 0.001 vs. 0 h; #P < 0.05 vs. 1000 ng/mL at same time point.

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