Fig. 2
From: Critical role of mitochondrial aconitase in skeletal muscle maturation
Diaphragm muscle fiber immaturity was observed in Aco2 cKO mice, (A): Results of HE staining of diaphragm cross sections from Ctrl and cKO mice. (B): Expression of Dmd, Myoz1, Myoz3, and Tnni2 was analyzed by RT-qPCR using RNA extracted from the right hind limb of Ctrl and cKO mice. The graph shows relative values compared to the Ctrl, presented as mean ± SD (Ctrl: n = 6, cKO: n = 5). (C): Fluorescent immunostaining was performed on diaphragm cross sections from Ctrl and cKO mice using Laminin α2 (red) and dystrophin (cyan). (D): Whole-mount staining of the diaphragm from Ctrl and cKO mice using dystrophin (cyan) and DAPI (blue). Z-stack images were captured at 0.7 μm intervals, and 3D images of the diaphragm surface approximately 70–90 μm deep were generated. The green and magenta boxes indicate the XZ and ZY views, respectively. Statistical analyses were performed using two-sided t-tests. The scale bars indicate 50 μm.
