Table 1 Primer sequences for gene detection by qRT-PCR.
From: METTL3 promotes vascular stability in intracranial aneurysm via m6A-AMPK axis
Genes | Sequence(5’-3) |
|---|---|
β-Actin (Human) | F: TCCTCCTGAGCGCAAGTACTCC |
R: CATACTCCTGCTTGCTGATCCAC | |
AMPK (Human) | F: CCTGCTTGATGCACACATGA |
R: TCTGGGCCTGCATACAATCT | |
METTL3(Human) | F: CTTTGCCAGTTCGTTAGTCTC |
R: TTCAGAAGGCTCTCTATCTCCA | |
β-Actin (Rat) | F: ACCCAGATCATGTTTGAGACCT |
R: GACCAGAGGCATACAGGGACAAC | |
METTL3 (Rat) | F: CTGGGCACTTGGATTTAAGGAA |
R: TGAGAGGTGGTGTAGCAACTT | |
AMPK (Rat) | F: CAGGAAGATTGTACGCAGGC |
R: AAAGAGTTGGCACGTGGTC | |
α-SMA (Rat) | F: GGCGGAAGTCGTCACTCTTA |
R: CAGAATCATCGCTCTGGCCT | |
SM-22α (Rat) | F: GAACTTCCAAGGAGCTTTCCC |
R: GCACTATGATCCACTCCACCA | |
IL-1β (Rat) | F: ACCTTCCAGGATGAGGACATGA |
R: AACGTCACACACCAGCAGGTTA | |
IL-6 (Rat) | F: AGTTGCCTTCTTGGGACTGA |
R: TCCACGATTTCCCAGAGAAC | |
TNF-α (Rat) | F: AAGCAAGCAGCCAACCAG |
R: TCTTCTGCCAGTTCCACG | |
MMP3 (Rat) | F: TCCCTTGATGCCATTACTAGTCT |
R: AAAACAGTTCAGGCTCAACC | |
MMP9 (Rat) | F: CCCAACCTTTACCAGCTACTC |
R: GTCAGAACCGACCCTACAAAG | |
iNOS (Rat) | F: TGCATGTGACTCCATCGACCC |
R: TGGACCCCATGCATAATTTGGAC |
