Fig. 4
Representative images of immunohistochemical staining for protein expression in GIT tissues of feedlot steers. (A) Claudin 1 (CLDN1) in the colon: moderate, diffuse cytoplasmic and membranous staining is evident in the epithelium, with weaker staining in the lamina propria. (B) Claudin 2 (CLDN2) in the rumen: no detectable immunopositivity in the epithelium or lamina propria; observed staining is attributed to nonspecific uptake by debris. (C) Occludin (OCLDN) in the jejunum: moderate, diffuse cytoplasmic immunoreactivity in the epithelium with minimal staining in the lamina propria. (D) Calprotectin (MAC-387) in the rumen: weak to strong nuclear and cytoplasmic immunoreactivity observed in the epithelium. (E) E-cadherin (CDH1) in the jejunum: strong cytoplasmic and membranous immunoreactivity within the epithelium, and weak staining in the lamina propria. Scale bars: A–D = 100 µm; E = 250 µm.
