Fig. 5 | Scientific Reports

Fig. 5

From: Microbial communities and tight junction protein expression in the gastrointestinal tract of feedlot cattle

Fig. 5

Pixelwise H-scores for expression of TJPs and an inflammatory marker in GIT tissues of feedlot steers based on computerized image analysis. Dot and Boxplots show H-scores derived from image analysis of immunohistochemical staining of tissue sections for Claudin 1, Claudin 2, Occludin, E-Cadherin, and MAC-387 (Calprotectin) in the rumen (RU ES), jejunum (SI ES), and colon (LI ES). H-scores were generated using pixel-level quantification algorithms from QuPath software. Within each plot, boxes with different superscripts (a, b, c) indicate statistically significant differences among tissue types (P < 0.01).

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