Fig. 8

Bland–Altman plots evaluating agreement between scoring methods of immunohistochemical staining of TJPs and an inflammatory marker in GIT tissues of feedlot steers (Claudin 1 = CLDN1, Claudin 2 = CLDN2, Occludin = OCLDN, E-cadherin = E-CAD, MAC-387 = Calprotectin). Plots compare the mean H-scores for samples generated by a panel of 3 pathologists for epithelial tissues (Epi H), lamina propria (LP), or a combined average of scores of the epithelium and lamina propria (AVG) vs the computer-generated pixelwise H-score (automated QuPath analysis) for the epithelium and lamina propria combined. Solid blue lines indicate mean bias, and red dashed lines represent the 95% confidence interval limits.