Fig. 4

(a) The dependence of the reaction velocity on the H₂O₂ concentration (the concentration of TMB was fixed at 166 μM). (b) The corresponding Lineweaver–Burk plot. (c) The dependence of the reaction velocity on the TMB concentration (the concentration of H₂O₂ was fixed at 314 μM). (d) The corresponding Lineweaver–Burk plot. The test conditions: 10 μL of 1 mg mL⁻¹ Mn₃O₄ nanozymes in 1 mL of 10 mM PBS (pH = 5.35) at 35 °C. The error bars were obtained based on three sets of measurement.