Fig. 3 | Scientific Reports

Fig. 3

From: Single-cell and bulk transcriptomics reveal Fusobacterium nucleatum promotes colorectal cancer metastasis by upregulating LAMC2 in in vitro and in vivo models

Fig. 3

F. nucleatum promotes metastasis of CRC cells in a LAMC2 dependent manner. (A and B) Detection of the knockdown efficiency of LAMC2 by different sequences of lentiviral vectors at the mRNA (n = 4 per group, One-way ANOVA test) and protein (repeated three times) levels. LOVO cell were transfected with LAMC2 shRNA lentivirus or control lentivirus, and then incubated with F. nucleatum or PBS. (C and D) The mRNA expression and protein expression of LAMC2 were measured by qPCR (n = 4, One-way ANOVA test) and western blot (repeated three times). (E and G) The motility was detected by wound-healing assay (n = 6, One-way ANOVA test) or (F and H) transwell assay(n = 5, One-way ANOVA test). (I) LOVO cells constructed with lentivirus were incubated with F. nucleatum or PBS and then injected into the tail vein of nude mice (5 mice per group). (J and K) HE staining represents the images and the number of metastatic lesions (Student’s t-test) in each group. Data are shown as mean ± SD. * represents p < 0 0.05, ** represents p < 0 0.01, *** represents p < 0.001, and **** represents p < 0.0001, ns represents no statistical significant.

Back to article page