Fig. 3
Dietary fiber reduces Aβ40 and Aβ42 deposition in 5xFAD mouse brain. Representative immunofluorescence images of Aβ species in brain sections from 6-month-old FFAD and FPAD mice. (a) Staining for total Aβ (red) and Aβ40 (green) in whole hemisphere, hippocampus, somatosensory cortex, and entorhinal cortex. (b) Staining for total Aβ (red) and Aβ42 (green) in the same brain regions. Nuclei were counterstained with DAPI (blue). Yellow boxes in hemisphere panels indicate regions magnified below. Scale bars: 300 μm (hemisphere), 50 μm (hippocampus and cortex). Quantification of Aβ burden and Aβ42/Aβ40 ratio is shown in Supplementary Fig. 4a. Data represent n = 5 mice per group, with four sections per brain region per mouse analyzed.
