Fig. 7
Fecal lipidomic profiles in WT and AD mice across diets and ages. Fecal lipids were extracted using a modified Folch method and analyzed via untargeted lipidomics. Bar graphs show the relative abundance of triglycerides (TG), lysophosphatidylcholines (LPC), phosphatidylcholines (PC), sphingomyelins (SM), phosphatidylethanolamines (PE), and diacylglycerols (DG). White bars with red circles indicate WT mice, and purple bars with blue circles indicate AD mice. At 1 month (1 M), WT and AD mice (n = 8/group) were analyzed while maintained on standard chow diet, serving as the baseline prior to dietary intervention. Mice were fed custom fiber-free (FF) or fiber-plus (FP) diets for 3 or 5 months (4 and 6 months of age), respectively (n = 3–4/group). Data are presented as mean ± SEM. Statistical significance was determined by two-way ANOVA (factors: genotype and diet) followed by Tukey’s post hoc test. *p < 0.05, with comparisons indicated by black connecting lines.
