Fig. 2

Comparison of oral microbial community structure (beta-diversity) between mild depression (n = 13) and major depression (n = 19) groups. Analyses included Unweighted UniFrac, Weighted UniFrac, Principal Co-ordinates Analysis (PCoA), Principal Component Analysis (PCA), and Non-metric multidimensional scaling (NMDS). The Unweighted UniFrac distance reflected differences in microbial presence/absence, while the Weighted UniFrac distance incorporated both phylogenetic composition and abundance. The first two principal coordinates (PC1, PC2) or multidimensional scaling axes (MDS1, MDS2) were shown, with percentages indicating the proportion of variance explained by each component. Statistical significance was assessed using permutational multivariate analysis of variance (PERMANOVA). PHQ-9, Patient Health Questionnaire-9; P, P value.