Fig. 1 | Scientific Reports

Fig. 1

From: Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system

Fig. 1

Scheme of transgene-free base editing system and all-in-one ARS base editor (ArBE) vectors. (A) Scheme of transgene-free and double-strand break (DSB)-free genome editing using ArBE vectors. gDNA: Genomic DNA; A: Adenine base; C: Cytidine base; G: Guanine base; T: Thymidine base. (B) Map of ArBE vectors. terLDSP: Terminator of the endogenous gene, LDSP; UGI: uracil DNA glycosylase inhibitor protein; nCas9(D10A): A Cas9 nickase with an aspartic acid-to-alanine substitution at the 10th aspartic acid residue; ProRibi: Bidirectional promoter; HH: Hammerhead ribozyme that has self-cleavage activity; sgRNA: single-guide RNA; HDV: Hepatitis delta virus ribozyme that has self-cleavage activity; terFCP: Terminator of endogenous gene, FCP; ShBle: Zeocin-resistance gene; ProLHC: Promoter of the endogenous gene, LHC; AmpR: Ampicillin-resistance gene cassette; ARS: Centromere and autonomous replication sequence (CEN/ARS).

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