Fig. 1 | Scientific Reports

Fig. 1

From: Tracking copper-zinc and manganese superoxide dismutase in Avicennia marina reveals time-dependent expression of SOD isoforms in response to salt and lead stress

Fig. 1

SDS-PAGE analysis, Purification and titration curve of produced antibodies. (A) Soluble protein extracted from R-tAmSOD2 (lane 1), and R-AmSOD1 (lane 2) and control strain (lane 3) after 4 h induction with IPTG. (B) SDS-PAGE analysis for confirmation of quality of His-AmSOD1 (lanes 1–4) and His-tAmSOD2 (lanes 5–8) after purification with affinity chromatography. (C) Titration curve of produced antibodies against recombinant and pure His-AmSOD1 and His-tAmSOD2 using ElISA. (D) Determination of specificity of produced antibody using western blotting.

Back to article page