Fig. 5
Comparison of gene content predicted for each sequence of the genome pan-assembly of B. henselae, E. coli , and P. aeruginosa. Based on gene content (presence: blue), a pan-genomic approach was used to assess all assemblies. Expectedgenes (based on the reference sequence) were found for most cases using short-reads, hybrid approaches with Unicycler and somehybrids with Wengan. Other Wengan-based hybrid assemblies, as well as long-read only cases, resulted in an unexpected profilewith a different number of genes and apparently several “exclusive” genes. Despite some exceptions, no association betweensequencing depth and the gene content outcome is evidenced.
