Fig. 5

Gating strategy for MSC-l cell sorting. (A) MSC-l cells expressing CD13^brightCD45^low/negCD105^posCD90^pos surface markers were isolated from low-density bone marrow samples of AML patients. (B) Brightfield images of culture growth of CD13^brightCD45^low/neg CD105^pos and CD90^pos MSC-l cells. The white arrows highlight cells displaying characteristic MSC-l morphologies, such as elongated spindle shapes and cuboidal or polygonal forms, demonstrating successful culture and growth of MSC-l cells. Scale bar 200 µm.