Fig. 6

EGCG regulates and improves the phosphorylation levels of proteins in the JAK2/STAT3 signaling pathway via its agonists and inhibitors. MLE-12 cells were inoculated in 6-well plates and grown to 60% confluence. The cells were incubated with Coumermycin A1, JAK-IN-21, Colivelin TFA, and Stattic in culture medium for 48 h. The medium was then refreshed, followed by a 24-h pretreatment with EGCG, and finally exposure to LPS for 10 h. (A–B) Protein levels of JAK2, p-JAK2, STAT3, and p-STAT3 were measured by western blotting. (C–D) Relative intensities of p-JAK2/JAK2 and p-STAT3/STAT3 were analyzed via ImageJ software. The data are presented as the means ± SEMs (N = 3, ^#### P < 0.0001 versus the control group; ** P < 0.01 and **** P < 0.001 versus the LPS-treated group; ^& P < 0.05, ^&&& P < 0.001, ^&&&& P < 0.0001 versus the LPS + EGCG 7.5 µM group).