Fig. 7

EGCG inhibited inflammation through the JAK2/STAT3 signaling pathway. MLE-12 cells were subjected to the experimental procedure shown in Fig. 6. After 48 h, the medium was refreshed, and the cells were subsequently administered with EGCG and LPS. (A–C) Levels of IL-1β, IL-6, and TNF-α in cell supernatants were quantified by ELISA. (D–F) Cellular levels of IL-1β, IL-6, and TNF-α were measured by RT–PCR. The data are presented as means ± SEMs (N = 3, ^#### P < 0.0001 versus the control group; *** P < 0.001 and **** P < 0.0001 versus the LPS-treated group; ^& P < 0.05, ^&& P < 0.01, ^&&& P < 0.001, and ^&&&& P < 0.0001 versus the LPS + EGCG 7.5 µM group).