Fig. 6

WGCNA for combined datasets. (A) Display of the scale-free network with the optimal soft threshold in weighted gene co-expression network analysis (WGCNA). The left plot shows the optimal soft threshold, and the right plot shows the network connectivity under different soft thresholds. (B) Display of the correlation analysis results between clustering modules of the top 3,000 genes with the highest variance and the Control and IDD groups. (C) Display of the module aggregation results of the top 3,000 genes with the highest variance. (D) Display of the Venn diagram of 25 GRDEGs and genes contained in the MEpink and MEgreen modules. (E) Correlation heatmap of expression levels between hub genes. IDD, Intervertebral Disc Degeneration; WGCNA, Weighted Gene Co-Expression Network Analysis; GRDEGs, Glycosylation-Related Differentially Expressed Genes. *Represents p-value < 0.05, indicating statistical significance; ** represents p-value < 0.01, indicating high statistical significance; *** represents p-value < 0.01, indicating extreme statistical significance. The absolute value of the correlation coefficient (r value) below 0.3 is weak or uncorrelated, between 0.3 and 0.5 is weakly correlated, between 0.5 and 0.8 is moderately correlated, and above 0.8 is strongly correlated. Red represents positive correlation and blue represents negative correlation.