Fig. 3
Knockdown of KDM6B promotes the enrichment of H3K27me3 in MFN1 promoter. A H3K27me3 levels in the lungs of mice in the sham and CLP group were measured using western blot, and H3 served as the internal control. B After KDM6B knockdown, H3K27me3 levels in Raw264.7 cells were examined by western blot, and H3 acted as the internal control. C H3K27me3 levels were observed using IF after KDM6B knockdown in Raw264.7 cells. D The mRNA expression levels of MFN1, MFN2, Drp1, and ATG7, as well as their protein levels were measured after KDM6B knockdown using qPCR and western blot, respectively. E The effect of KDM6B knockdown on the enrichment of H3K27me3 in the promoter of the MFN1 gene was evaluated using CHIP. ***P < 0.001 vs. the shNC group. ns: no significance vs. the shNC group.
