Fig. 5
From: Extracellular enzymatic activities of octocorals and scleractinian corals under environmental stress
Experimental design for extracellular enzymatic activities (EEAs) measurement. (1) First experiment: Release of EEAs (APA, LAP, ⍺-Glu) by the mucus-associated microorganisms in corals (Sarcophyton, Lobophytum, Stylophora and Turbinaria) maintained in control condition (n = 6 per species) and FSW (n = 3) as a control for any non-enzymatic hydrolysis of the substrate. (2) Second experiment: Effect of temperature and low light on APA, LAP, ⍺-Glu in corals (Sarcophyton, Lobophytum, Stylophora and Turbinaria) (2.a; n = 6 per species and condition) and on their physiology (2.b; n = 6 per species and condition). (3) Third experiment: Effect of a depth gradient on the APA in corals (Sarcophyton, Rythisma, Xenia, Stylophora, Turbinaria and Galaxea) on the field (IUI, Red Sea) (n = 6 per species and depth; no data collected for Xenia from mesophotic depth). Substrate was added at a final concentration of 250 μM in each beaker. APA, alkaline phosphatase activity; FSW, filtered seawater; LAP, leucine-aminopeptidase; Leu-AMC, Leucine-7-amido-4-methylcoumarin hydrochloride; p-NPP, para-nitrophenyl phosphate; α-Glu, ⍺-glucosidase; α-MUF, 4-methylumbelliferyl-α-D-glucopyranoside. Sarcophyton., Sarcophyton glaucum; Lobophytum., Lobophytum sp.; Stylophora., Stylophora pistillata; Turbinaria., Turbinaria reniformis; Xenia., Xenia sp.; Rythisma., Rythisma fulvum; Galaxea., Galaxea fascicularis.
