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Fig. 1

From: Streptococcus gordonii type VII secretion system substrate EsxA induces neutrophil extracellular trap formation in infective endocarditis

Fig. 1

EssC mediates EsxA secretion in the S. gordonii DL1. (a) Comparison of the S. gordonii type VII secretion system gene cluster with that of Streptococcus agalactiae, Streptococcus gallolyticus, Staphylococcus aureus and Listeria monocytogenes. (b) Homology tree of EssC amino acid sequences from pathogenic Firmicutes. (c) Alignment of EsxA amino sequences obtained from the NCBI database. (d) Homology tree of EsxA amino sequences from pathogenic Firmicutes. The EssC and EsxA amino acid sequences had a high degree of sequence conservation. (e) Wild-type S. gordonii DL1, ΔessC, ΔesxA, compΔessC and compΔesxA strains were grown in BHI broth and resuspended in sample lysis buffer to obtain a total bacterial protein extract. Supernatant proteins were precipitated with 20% TCA buffer and resuspended in sample buffer. EsxA was detected by western blotting with anti-EsxA antibodies. Uncropped blots are provided in Supplementary Figure S1 and S7. The experiments were performed three times, and representative results from one experiment are shown.

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