Table 1 PCR primers used in this study.

From: Streptococcus gordonii type VII secretion system substrate EsxA induces neutrophil extracellular trap formation in infective endocarditis

Primers

Sequence (5’ to 3’) (restriction sites: underlined)

Target

For generation of isogenic mutant and complementation strains

esxA_BF

ATCCATCGTATCGCTTCAAA

up stream fragment of esxA

esxA_BR_EcoRI

CCGGAATTCGCAATTCTTCTGGAGTTA

esxA_AF_EcoRI

CCGGAATTCTTGCAGCACAAATTCATTA

down stream fragment of esxA

esxA_AR

ACTGTTCACATCCAGTACTTT

essC_BF

AATGTGAGTCTTAAATCGGA

up stream fragment of essC

essC_BR_EcoRI

CGATCGGAATTCTTGCTTTACAGGAGTATCGA

esxC_AF_EcoRI

CGATCGGAATTCTTGCTTTACAGGAGTATCGA

down stream fragment of essC

essC_AR

ATCATCAGAAGTCAGCGG

esxA_EcoRI

GCCGGAATTCGGTAAATGAAGGCATCT

complementation of esxA

esxAR _EcoRI

ACCGGAATTCTTAATGATGATGATGATGATGAATTTGTGCTGCAATATC

essCF_BclI

CGATGCTGATCAATGACAAAACAAGTAATTTTCT

complementation of essC

essCR_BclI

TATGGCTGATCATTATTCTACTCCTTTCACTAA

KanF

CTCGGAATTCCAAAAGAGGAAAGGAAATAATAAATG

Kanamycin gene

KanR

GACGGAATTCCTAAAACAATTCATCCAGTAA

For construction of recombinant EsxA

rEsxA-BamHI F

TAGCGCGGATCCATGGCTCGGATTAAATTAAC

rEsxA

rEsxA-BamHI R

CTACGCGGATCCTTAATGAATTTGTGCTGCAAT

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