Fig. 5
From: ASS1 facilitates T-ALL progression via the arginine-mediated mTORC1/c-Myc signaling pathway
ASS1 couples arginine metabolism to mTORC1/c-Myc activation. A. c-Myc expression levels in T-ALL cell lines versus normal T cells. B. Correlation analysis of ASS1 and c-Myc protein expression in T-ALL and normal T cells. C-E. Protein levels of ASS1, p-S6, and c-Myc in ASS1-knockdown (C) Jurkat, (D) MOLT-4, and (E) KE-37 cells. T-ALL cells were collected at 72 h after transfection using lentivirus vectors, using non-target shRNA (shC) as negative control. F. Intracellular arginine quantification in ASS1-knockdown Jurkat cells. Jurkat cells were first transfected with the lentiviral vector carrying shASS1. After 72 h, the cells were cultured in arginine-free medium for 24 h, and then the intracellular arginine levels were quantified. G. mTORC1/c-Myc pathway activity and (H) cell proliferation of shC control and ASS1-knockdown Jurkat cells with or without arginine (500 µM) supplementation for 48 h. Data are mean ± SD (Two-tailed unpaired Student’s t-test, ** p < 0.01).
